Sentence examples for purification of expressed from inspiring English sources

An expression vector system has been developed, taking advantage of a novel, Staphylococcus aureus protein A (SPA -binding affinity tag ZSPA -bindinging straffinityward affinitagblotting procedures and efficient recovery by affinity purification of expressed gene products on readily available reagents and chromatography media.

The purification of expressed protein is usually the bottleneck in the cost of protein production and this has put immense pressure on the need to develop novel purification methods and make improvements to existing strategies.

Since expression in E. coli culture resulted in accumulation of the recombinant protein in insoluble "inclusion bodies", purification of expressed chimera was performed by washing the pellets using a low urea concentration, followed by a high urea concentration for the extraction (solubilization) processes.

The pSin1 vector similarly contained sequences encoding a MYC-tag, facilitating detection with the murine monoclonal antibody 9E10 (ATCC CRL 1729), and a His6 tag, allowing purification of expressed proteins by immobilized metal-chelate affinity chromatography (IMAC) and detection using a murine Penta-HIS antibody (Qiagen).

The functional analysis of genes typically requires each ORF to be over-expressed or silenced, the purification of the expressed protein, production of antibodies, analysis of phenotypes, determination of intracellular localization, and analysis of interactions with other proteins.

We constructed a 5xMyc-BI-1-expression plasmid, allowing the detection and purification of ectopically expressed BI-1 from transfected HeLa cells using anti-Myc-agarose beads.

To facilitate purification of the expressed complex, we have prepared a suite of 72 plasmids which allows individual subunits to be tagged at the N- or C-terminus with six permanent or cleavable peptide affinity tags.

The established glutathione S-transferase (GST -capture ELISA is based on a one-step purification of bacterially expressed antiGST -capture dELISAed to detect serum antisodies against L1 capsid proteins of Mastomys natalensis papillomavirus (MnPV) and M. coucha papillomavirus 2 (McPV2).

Purification of the expressed SBP-Cap∆41 fusion protein was by immobilized metal affinity chromatography using the His-Bind Purification kit (Novagen, USA) according to the manufacturer's instructions.

Since the performance of whole cell biotransformations involves disadvantages like for instance transport limitations and low substrate solubility in aqueous systems, the isolation and purification of recombinantly expressed proteins is often advantageous.

Purification of the expressed protein was carried out as explained earlier.