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Primary Treatment: The sifted shit is then pumped into a container where it stays until gravity separates any remaining grit and deemed safe for the purification machinery.
Primary Treatment: The sifted shit is then pumped into a container where it stays until gravity separates any remaining grit and it is deemed safe for the purification machinery.
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A single ion-exchange chromatography was performed for the one-step purification of the soluble folding machineries.
For efficient and simple purification and immobilization simultaneously, folding machineries were fused with the positively-charged consecutive 10-arginine tag at their C-terminal.
Therefore, a cost-efficient purification and immobilization of folding machineries is a critical step in order to apply this refolding methodology to a large bioprocess scale.
In this study, we report a simple method that enables simultaneous purification and immobilization of folding machineries in fully functional forms.
Here, we report a simple method that enables one-step purification and immobilization of folding machineries (mini-chaperone, DsbA and human PPIase) simultaneously in fully functional forms using the positively-charged consecutive 10-arginine tag at their C-terminal.
Biochemical purification or reconstitution or structural characterization of machinery containing a large number of subunits can be a challenging proposition.
Purification and analysis of the putative dynein plus-end transport machinery.
(A ) Interactions between core components of the CIA machinery (i.e., Tah18, Dre2, Cfd1, Nbp35, Nar1, Cia1, Cia2 and Mms19) identified by TAP-MS (tandem affinity purification (TAP) coupled to protein identification by mass spectrometry) with additional STRING evidence.
Machinery malfunctioned.
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