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Water purification is provided by the Namibian Red Cross.
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The details of the purification are provided in the Supporting Information.
Details of OPA1-S1 purificareon are provided in Supplementary Material, Text S1.
Results of F3 empty FRET and ABAleon purifications are provided in Figure 7 figure supplement 2. The coding sequence of ΔNABI1 corresponding to amino acid residues 125 429 was inserted via Nde I/ Bam HI into pET28a (Novagen; Supplementary file 1A, Supplementary file 1B) and transformed into E. coli BL21 (DE3).
An overview of the in situ purification of these proteins is provided in Table 1.
In this study, an extensive review of separation and purification of butanol from fermentation broth is provided, including gas stripping, vacuum flash, liquid liquid extraction, membrane solvent extraction or perstraction, membrane pervaporation, membrane distillation, thermopervaporation, reverse osmosis, adsorption, and integrated bioprocessing with various separation methods.
Detailed description of reagents, peptide synthesis, molecular cloning, protein expression and purification, and mass spectrometry sample preparation is provided in the Supporting Information.
All the buffers and the phosphopeptides purification resin were provided in the kit by the manufacturer.
Plasmid DNA purification kit was provided by EURx (Gdansk, Poland).
Details of the purification methods are provided in the Supporting Information and are summarized in Table 1.
In addition, an improved appearance of purification sites can be provided by using ornamental plants with many flowers, as shown in Figure 4(c).
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