Exact(1)
Further purification conducted by liquid-liquid partition separated the root crude extract into four fractions based on the different solvent polarities.
Similar(59)
RNA purification was conducted using the RNA purification column with conditioning buffer and 70% ethanol.
Virus purification was conducted using an adenovirus purification kit (Biomiga, USA), according to the manufacturer's instructions.
Purification was conducted using the IMAC purification kit on Profinia (Bio-Rad), yielding 87 ± 5 μg of purified protein/L of culture medium.
GAG liberation and purification was conducted as described for guanidine-extracted PG with the exception of an additional purification step as follows to remove contaminating DNA in the tissue extracts: GAG-ethanol precipitates were solublized in 500 μl of dH2O, adjusted to a final concentration of 10% trichloroacetic acid, 0.01% BSA and incubated on ice for 1 hour.
Brief protein purification was conducted using Ni-NTA affinity chromatography.
DNA extraction, PCR amplification, and amplicon purification were conducted as described elsewhere (Miyahara et al. 2013).
The correlation coefficients were obtained based on the results of photocatalytic air purification experiments conducted.
Further analysis and purification was conducted using RP-HPLC method (Waters Symmetry C18 column, 250 × 4.6 mm, 5 μm, Dublin, Ireland) with a gradient separation (mobile phase B: 5 100%) at a flow rate of 0.5 mL/min at 25 °C.
Further purification was conducted by washing the sediment several times with a small amount of ethyl acetate, and the sample was dried with nitrogen blowing and then freeze-dried to a constant weight.
The semi-preparative HPLC purification was conducted on Phenomenex Kinetex® 5 μm 150 × 10 mm column and mobile phase of 40%% MeCN in 20 mM NH4HCO3 with flow rate of 5 mL/min.
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