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Its compact, lightweight filters kill bacteria and parasites in a purification cartridge containing millions of tiny cleansing pores.
Contents of one reaction vessel can be transferred to another cassette, the HPLC valve, or to a purification cartridge.
The purification cartridge is installed between the dip tube (for removal of crude product from the reaction vessel) and the tube leading to the stopcock.
The C18 purification cartridge is conditioned with 2 ml 70% (V/V) ethanol, purged with 1 ml air and subsequently rinsed with 5 ml sodium chloride (NaCl) 0.9%.
Push with 10 psi inert gas for 30 s 15 ADD Add Water-1 to reactor 1 16 TRANSFER Transfer the contents of reactor 1 through the purification cartridge to the collection vial.
Besides the test for the potential introduction of bioburden and endotoxins from the generator, a validation study was performed to assess the total bioburden and possible introduction of bacterial endotoxins from non-sterile DOTA-NOC acetate, the 68Ga radiolabelling kit and C18 purification cartridge.
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Sep-Pak purification cartridges (Sep-Pak), e.g., silica, C18, etc., are connected to designated Luer fittings on the cassette.
Each cassette contains three stopcock valves and has a dip tube for transferring products to purification cartridges, subsequent cassettes, or the HPLC injection valve.
After deprotection with HCl (unit operations 12 to 13), the crude product was purified (SCX, ion retardation, Alumina-N, and C18) using the TRANSFER unit operation (unit operation 14) and subsequently rinsed (twice) by adding water to the reaction vessel and transferring it through the purification cartridges (unit operations 15 to 18) and a sterile 0.22-μm filter into a sterile vial.
Acyl-CoAs were purified using Oligonucleotide Purification Cartridges (Applied Biosystems, Foster City, CA) and eluted with 60% acetonitrile.
Amplification products were separated from unused primers by using QIAquick 96 PCR purification cartridges (Qiagen, with modified binding and wash buffers).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com