Sentence examples for purification approach from inspiring English sources

For every purification approach, 1 mL of this prepared bead suspension was incubated with 4 mL crude cell extract for 60 min in an overhead shaker at 4 °C.

Therefore, a simple 19F/18F substitution reaction was attempted, and >80% RCY of [18F] ESF was obtained in diluted saline at 130 °C, employing a simple alumina cartridge purification approach (Fig. 8).

The double-tagged variant of Cel5A was purified to apparent homogeneity (Additional file 1: Figure S2A), while a purification approach using Bgl1 led to the detection of an additional, smaller HIS-tag protein (Additional file 1: Figure S2B).

The cadA gene was identified via a clone-based transcriptomics approach (Li et al. 2011) and via an enzyme purification approach (Kanamasa et al. 2008; van der Straat and de Graaff, personal communications).

It has to be noted that every purification approach using metal ion affinity was compared with other works using IMAC chromatography for purification instead of batch approaches, since no such approaches have been reported yet.

To address the need for tools that facilitate cell-type-specific epigenomic studies, we developed the first affinity purification approach to isolate nuclei from genetically defined cell types in a mammal.

Cell pellets were frozen at −80°C for storage and further used for purification approaches.

The hydrophobic characteristics of the lipidated peptides again, result in specific synthesis and purification approaches for the entire lipidated proteins.

Using biochemical purification approaches, we previously verified that YY1 is tightly associated with the human INO80 (INO80) chromatin remodeling complex (Jin et al. 2005).

The result was that with so many upstream strategies available, the classical purification approaches were perceived to be slow, inefficient and on the whole inadequate.

Since the purification approaches in this work already showed a decreased enzyme activity for the isolated d-Hyd(co) compared to the immobilized enzyme, it was suggested that occupation of the His-tag by immobilization employing this tag results in shielding of these histidine residues from the catalytic center of the enzyme.