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These two-phase flow effects have been studied at relatively low operating temperatures under steady-state conditions and during transient air purging sequences.
Considering that a majority of erroneous sequences are single nt substitutions, it should be possible to purge erroneous sequences by excluding sequences present at lower frequency and differing by a single nt from an index sequence.
The rarity of large intergenic regions in bacteria has been explained by an observed excess of deletion mutations relative to insertions, which purge nonfunctional sequence from the genome (Mira et al. 2001).
As each sample analyzed for CDR repertoire possesses variable intrinsic diversity, it is not possible to a priori define the extent to which specific interventions that exclude sequences from a data set will mistakenly purge true sequences.
Increasing the threshold value for filtering potentially incorrect sequences may eliminate more erroneous events but will also increasingly purge true sequences.
According to the mutational-hazard hypothesis, genes in mutational hot spots have higher hazards and thus experience stronger selective forces to purge surplus sequences.
Organisms with a larger Ne are expected to purge noncoding sequences more effectively than those with a smaller Ne (Lynch and Conery 2003).
The growth cycle consists of precursor exposures and N2 99.9999%) purge following the sequence of DEZn/N2/H2O/N2 with corresponding duration of 0.1 5 0.1 5 s.
Selection may purge from cryptic sequences those variants whose expression is strongly and unconditionally deleterious, even when the sequences are expressed only at low levels via molecular errors.
Where their density is relatively low it is assumed that there is a greater likelihood that additional insertion or deletion variants have been preferentially purged in functional sequence.
All sequences were subsequently purged for simple sequence motifs with the help of Sputnik http://espressosoftware.com/sputnik/index.html and individual haplotypes were resolved with PHASE v2.1 [ 46, 47].
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