Sentence examples for pure template from inspiring English sources

To confirm the identity of the species studied, DNA was obtained from the isolates (High Pure Template Prep. Kit Roche Diagnostics Indianapolis IN), PCR amplified using primers for IS 900 (MAP) [24]and IS 901/2 [25] (M. avium & specific subspecies including silveticum) as described.

DNA was extracted using the Roche High Pure Template kit as per manufacturer's instruction.

cfDNA was isolated out of 400 μl patients' serum using Roche High pure template preparation kit (Roche) with a modified isolation protocol published previously Wielscher et al., 2011.

DNA isolation from serum using common silica membrane-based isolation strategy was performed applying the Roche High pure template preparation kit (Roche Diagnostics, Mannheim, Germany).

Purified culture was used for genomic DNA extraction according to High Pure Template Preparation Kit package insert (Roche Diagnostics, Indianapolis, IN, USA).

DNA was extracted from semen and cauda epididymidis samples for PCR analysis using a High Pure Template Preparation kit, following manufacturer's instructions (Roche Diagnostics, Basel, Switzerland) and stored at -20°C.

Notably, we find that when a thin pure "templating" layer of ClAlPc is grown first, the subsequently grown ClAlPc C60 bulk HJ propagates the templating effect, and films show a higher crystallinity than without this templating layer, with higher fill factors as a result.

Partial nox sequencing was performed on 83 successfully sub-cultured isolates, and high quality sequence consistent with pure templates was obtained for 81 of these [Genbank: KC561373-KC561453].

5′-P-labeled primers (50 pmol) were mixed with 2 equiv of HPLC-pure template strands (5′-TCA TXG AAT CCT TCC CCC-3′ where X = the click reaction generated covalent cross-link from the C-5 position of dU to the C-terminus of 6×His-eGFP, and N-terminus of 23-mer peptide (PDAQLVPGINGKAIHLVNNESSE), or 10-mer peptide (EQKLISEEDL)) in 10 mM Tris buffer (pH 7) containing 50 mM NaCl.

With a theoretically better probe chemistry and a purer template, SD and Ct values did not improve.

For the first PCR reaction 1 μg of DNA extracted from fecal samples with High Pure PCR Template Preparation Kit (Roche Diagnostics GmbH, Mannheim, Germany) was used as a template.