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Multiple Campylobacter isolates from a pure plate were pooled and inoculated into three, 1.5 mls of trypticase soy broth supplemented with 15% sterile glycerol and frozen at −70°C.
Subsequently, the two groups are tested for their preference between A versus B. For aversive learning larvae were tested on a salt plate, for appetitive learning larvae were tested on a pure plate [1].
Immediately after training, larvae were tested for five min for their preference of odor A or odor B. Aversive learning was tested on a salt plate and appetitive learning was tested on a pure plate – as published before [1], [2].
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When Cu 2 + : S 2 O 3 2 − mole ratio was varied to 1: 1 and 1: 1.5, phase pure plate-like natrochalcite [NaCu2 SO4)(H2O)] and digenite (Cu9S5) were produced respectively.
For total count of bacteria, 1 ml of each dilution was pure plated in a plate containing PCA medium (Merck GmbH, Darmstadt, Germany) and was incubated at 35 °C for 48 h and the number of colonies on each plate with 30 300 colonies were counted.
The pure phase plates (PPE) simulated by this method homogeneously concentrate more than 97% of the incident energy into the desired focal-plane region.
For example, Shiue et al. (2004a, b) reported the microstructure and properties of joints between commercial pure titanium plate and oxygen-free copper plate by infrared brazing.
If no separate colonies grew on the pure culture plate, N. gonorrhoeae colonies were harvested from a tertiary plate.
A 5 cm × 8 cm pure nickel plate was used as the auxiliary electrode.
The OCP values of coatings were much lower compared to pure Al plate.
The dramatic increase in the contrast-ratio and dynamic range was made possible by the newly engineered Pure Contrast Plate technology that effectively blocks unwanted light from the path of projection.
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