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Secondary goat anti-mouse antibody and streptavidin conjugates were from BD Pharmingen and pure mouse IgG1 was purchased from Caltag.
When M5-expressing bacteria were incubated in mouse serum, i.e. in the absence of Fg, complement was deposited on the bacterial surface, but addition of pure mouse Fg at physiological concentration caused an ∼6-fold reduction in deposition (Figure S2 and Methods S1).
We first tested the hybridisation of pure mouse non-engrafted spleen (NES) to human arrays.
MT transcripts increased in pure mouse astrocyte cultures upon contact with a microglia-conditioned medium.
The most promising hybridomas were cloned, and cell lines obtained that secreted pure mouse anti‐Tasmanian devil antibodies.
In retrospect, this finding may account for historic difficulties in obtaining pure mouse HSC populations and the low yields accompanying more promising approaches.
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Pure mice strains have been generated using brother/sister mating since the beginning of the twentieth century and have distinctive features as regards, reproductivity, behavioral characteristics and predisposition to specific diseases.
Hence, six cycles of ischaemia and reperfusion as an ischaemic postconditioning cardioprotective strategy in the isolated mouse heart model subjected to ischaemia reperfusion reduced the infarct size in C57BL/6 pure mice, but failed to protect in C57BL/6 floxed signal transducer and activator of transcription (STAT-3) mice commonly used as littermate controls in studies with STAT-3 knockout mice.
Charles Clitterhouse decides the difficulty is with his style--pure, unadulterated mouse--when what is wanted is incisive, decisive mouse-trap style.
Further studies in pure cone mouse models, the Rpe65−/−/Nrl−/− or Rpe65−/−/Rho−/− mice, will be necessary to identify the intracellular apoptotic pathways involved in the demise of the cones.
DNA (5 ng/L) was injected into the pronucleus of pure C57/Bl6 mouse embryos that were subsequently transferred into day 1-plugged pseudopregnant C57/Bl6 female mice.
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