Despite attempts to generate pure length-defined heparin oligosaccharides, chromatographic preparations represent a heterogeneously sulfated population of oligosaccharides, thus obscuring the critical structural features of HS/heparin required to inhibit different angiogenic cytokines.
In this section, we briefly review related work for improving delay performance of back-pressure scheduling, which can be roughly divided into the following three categories based on their design objectives: The first category aims at avoiding unnecessarily long or looped route which is often brought by back-pressure's pure queue length differential-based scheduling strategy.
Pure CAG length is not the only factor determining repeat instability.
In our hands, optimized conditions for splinted ligation with T4 DNA ligase have allowed for the production of pure, full length (232 nucleotides), spin-labeled riboswitches in yields satisfactory for several EPR and biochemical control experiments.
An N-terminal Glutathione-S-Transferase tag was used to promote soluble expression and was combined with a C-terminal hexahistidine tag to facilitate a simple non-denaturing two-step affinity chromatography procedure that results in highly pure full-length H1.4.
Purification of the cell lysate with Protein A was insufficient to give pure full-length IgG with bacterial proteins and heavy chain fragments co-purifying in the eluate and as a result subsequent Protein L purification was required.
Since we wanted to include all of the protein in our analysis, we developed an expression system for large quantities of pure full-length Xenopus CKIε fused to GST (CKIε-GST; see Methods).
Our initial attempt to obtain pure full-length hCaMKP-N using transfected Sf9 cells failed due to proteolysis during the expression and purification.
The fraction containing pure full-length PAD2 was concentrated using a Centricon concentrator with a 10 kDa nominal molecular mass cutoff.
Pure, full-length nucleic acid free coilin was purified as previously described (Broome and Hebert, 2013), except that after electroelution the protein was put through an SDS removal column (Thermo Scientific, Rockford, IL, USA) previously washed with high salt (250 mM NaCl) PBS.
