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The biological hydrogen production rate by the pure isolate (designated as Clostridium tyrobutyricum JM1) was dependent on various nutritional and environmental conditions.
The pure isolate was used for further steps.
The pure isolate was identified using biochemical methods [18], and 16S rRNA analysis.
Each pure isolate was then cultivated and placed into a Nunc® cryotube at minus 80 °C with glycerol (20 %) as cryoprotector.
Pure isolate F-7 and band 9 were relatives of Cryptococcaceae.
Pure isolate B-7 and band 7 were identified as Moraxellaceae and Lactobacillaceae, respectively.
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All the data were compared with the pure isolated single-walled SiC nanotube.
Interestingly, compared to pure isolates, the consortium degraded PFF more efficiently.
Several studies have highlighted that medicinal plants either as crude extracts or as pure isolated compounds can meet these criteria.
If you revere outsider artists as pure, isolated, often insane visionaries who exist outside time and place, make way for a so-called outsider whose work reflected many of the specifics of his cultural and historic moment.
Table 4 Identities of pure isolates Strain Closest relative Identity GenBank accession no.
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