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Therefore, we have also analyzed a very pure fraction of CD105+-hMSC derived from human CB, which has been purified by an immunomagnetic isolation technique.
In addition, the definitive application of a pure fraction of CD105+-hMSC from CB revealed overall a better myocardial performance than the whole proportion of CB derived hMSC and was similar to that of the MI-BM group.
Total RNA was isolated from pure fraction of peritoneal macrophages using the standard acid guanidinium thiocyanate-phenol-chloroform method.
Unlike chromosome sorting, laser microdissection is more laborious but produces a pure fraction of selected material [ 30, 31].
An evaporated pure fraction was sent for the structure analysis at Department of Chemistry, Faculty of Science, Chulalongkorn University.
In order to obtain a pure fraction of epimer 2, a second HPLC-separation had to be performed.
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Fractions were analysed by SDS-PAGE and pure fractions concentrated by centrifugation in Vivaspin 10,000 MWCO (Sartorius, Goettingen, Germany) proceeding crystallisation.
Pure fractions were selected based on the corresponding product signals in electrospray ionization mass spectrometry using direct injection on an Agilent 6520 Q-TOF LC-MS.
The crystal patterns are different from those of the two pure fractions.
The pure fractions obtained in chloroform from column chromatography were analyzed for their maximum wavelength i.e. λmax.
The mechanical segregation using only the force of gravity was not enough to obtain sufficiently pure fractions of the target particle size.
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