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RNA was extracted with the High Pure Extraction Kit (Roche, Indianapolis, IN, USA), according to manufacturer's instructions.
If the almond oil is not a pure extraction, it will fall flat.
The rest is a pure extraction of peppery watercress and basil.
In fact, SeptiFast with MagNA pure extraction closely resembles blood cultures, but presents discrepancies with the manual extraction test.
and extraction time (34 min).. To test the clinical utility of SeptiFast with MagNA pure extraction, an observational prospective study of 72 patients was carried out.
In sum, the results show that MagNA pure extraction followed by LightCycler SeptiFast detection was valid under the standard conditions tested and constitutes a plausible alternative to conventional manual extraction.
Similar(49)
DNA was robot-extracted (MagNA Pure Compact Extraction Robot; Roche, Basel, Switzerland) from 400 µL of EDTA-plasma (Nucleic Acid Isolation Kit I; Roche) and analyzed by using a real-time PCR specific for a 169-bp segment of the groEL gene of Candidatus N. mikurensis.
Tumour cells were captured in CapSure Macro LCM caps (Arcturus, Mountain View, CA, USA) and DNA was extracted using Pico Pure DNA extraction kit (Arcturus).
Depending on the pH value of the aqueous phase, pure physical extraction (pH ≤ 1) or reactive extraction (pH ⪢ 1) takes place.
MagNa pure® extraction versus the standard procedure for extraction used in the SeptiFast® test reduces the total test time from 6 to 3 or 3.5 hours.
Total RNA was extracted using the Ultra Pure RNA extraction Kit (Roche).
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