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Exploiting the finding that the enhancer landscape better reflects cell identity than mRNA levels, we enable 'enhancer cytometry' for enumeration of pure cell types from complex populations.
These technologies allow scientists to analyze relative abundances of key cellular signaling proteins from pure cell populations.
Using mass spectrometry on isolated high pure cell membrane fraction, we generated comprehensive cell surface omics for proteins (proteomics), lipids (lipidomics) and glycoproteins (glycomics).
Exploiting the endogenous fluorescent properties of the hNET substrate ASP+, it was possible to simultaneously, and efficiently, sort pure cell populations and evaluate reporter function.
HepG2 cells are a pure cell line of human liver carcinoma derived from the liver tissue with a well-differentiated hepatocellular carcinoma [15].
As determined by flow cytometry, the procedure resulted in >90% pure cell isolates.
This technological hurdle has been overcome and we will soon be able to replicate this paper with pure cell populations.
Because of the considerable heterogeneity of cells in and around tumor sites microdissection allowed us to harvest pure cell populations.
Similar(3)
Two contrasting finite volume schemes are described: a hybrid cell-vertex scheme and a pure cell-centred counterpart.
Using our estimated parameters, the pure cell-to-cell infection is simulated in silico (solid curves).
Flow sorting of Jurkat cells resulted in almost pure cell-cycle populations (Table 2).
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