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In transport experiments, many pups were obtained from vitrified/warmed 2-cell embryos transported at a cold temperature in all receiving laboratories (incidence of successful development: 49%; 249/511).
In this study, 61 and 14 pups were obtained from embryos cultured for 24 h or 96 h under µG conditions, respectively.
E18.5 pups were obtained from timed pregnancies in which a pregnant female was euthanized by CO2 inhalation followed by cervical dislocation.
Sprague-Dawley rat pups were obtained from C harles River Laboratories (Wilmington, MA, USA).
H2B-GFP colony was maintained in hemozygous conditions and WT or H2B-GFP transgenic pups were obtained from the same litter.
The naïve pups were obtained from our routine breeding scheme in which one C57BL/6 SR/CR parent is crossed with a C57BL/6 WT parent.
Similar(53)
But no pups were obtained.
No viable EpCAM −/− pups were obtained.
For the EGFRL858R+T790M transgene, 60 pups were obtained.
Thirty-seven founder pups were obtained by pronuclear microinjection, of which three were positive for the transgene.
To ensure sampling of female-pup pairs, biopsies were obtained from females only when they were nursing a marked pup.
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