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At that time, we investigated perigonadal adipose tissue of pups (weight, histology, gene expression).
Five parameters were taken into account: 1) placenta weight, 2) pups weight, 3) pre-implantation loss, 4) post-implantation loss; and 5) offspring vitality.
However, the observed AChE inhibition was not associated with any sign of systemic toxicity and/or weight loss in pregnant females or altered reproductive performances (fetus viability, number of pups delivered, sex ratio and mean pups' weight at birth).
Be sure to discuss proper feeding amounts and your pups weight with your veterinarian at your puppy regular visits.
Monitoring your pups weight and appearance can help to make sure you are feeding the appropriate amount.
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In each litter, the pups were separated into two groups according to birth weight: control pups and severely growth restricted (GR) pups weighting less than 5.50 g (6.34 g–2 S.D).
To minimize the differences in the pups' weights, one mouse nursed 6 8 pups, and low-weight pups were discarded from the data sets.
Percentile ranges of pups' weights (7.6 - 12.5 kg) are similar to values given in common literature, such as Jefferson et al.[ 49] or Burns [ 2].
Pup weight and systolic blood pressure are expressed as mean ± standard error of the mean and compared by using the unpaired t test.
We assessed the number of implantation scars, number of pups born, sex ratios of pups, average pup weight at birth and 2- and 6-weeks of age, and the survival rates between implantation and 2-weeks of age for two diet treatments.
The following variables were evaluated: proportion of animals that were delivered on day 23, time to delivery of the first pup (midnight on day 22 was considered to be 0 hour), number of stillborn pups, and average pup weight of each litter.
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