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In serum of the pups, levels of tumor necrosis factor (TNF), nitric oxide (NO), total antioxidant capacity (TAC) and malondialdehyde (MDA) were measured.
In NB1 pups, levels of all measured proteins were similar between groups, although small but significant decreases in caspase 3 and 8 were seen in the NB1 OSDM.
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To further clarify this hypothesis, we should examine breast milk levels and/or suckling pup plasma levels of DEHP and/or its metabolites and the time course of cytokine expression in our model, as well as in a model using oral administration of DEHP.
In addition, phase 3 pups demonstrated increased levels of reverse maze activity and a shorter and narrower gait.
Antibody staining further revealed that the full term pups had higher levels of three proteins whose expression is induced by conditions of oxygen deprivation, reflecting the fact that they had just been born.
In rodent studies, mouse pups receiving high levels of maternal care show increased neurotrophin levels in the brain, which promotes sociability in adulthood[7].
We showed that DAP12KI microglia cultured from P0 pups express higher levels of inflammatory markers such as NOS-2, IL1β, and IL6, as compared with their WT counterparts.
Such a pattern could arise through variation in resource allocation or acquisition between sea lion pups with different levels of heterozygosity [ 57].
Enard et al. [ 42] reported that when human-specific coding changes were engineered in mice (partially 'humanizing' them at the locus), this resulted in an altered structure of innate pup vocalizations, decreased levels of exploration, decreased levels of dopamine in the brain and an increased dendrite length and synaptic plasticity in the striatum.
Transmissions of immunity to pups were assessed by measuring the levels of immunity of pups from vaccinated and unvaccinated mothers.
This analysis demonstrated that while HNF4α levels increased during early postnatal development (Figure 1D), the sCre pups had considerably lower levels of functional HNF4α transcripts compared to the Cre or Cre mice throughout this time period, consistent with their observed phenotype.
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