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None of the reproductive parameters were affected and there were no effects on the offspring other than a small reduction in pre-weaning body weight gain of the F2 pups at the highest dose level.
Different sharks, like the blacktip reef shark that recently gave birth to pups at the aquarium, are viviparous; meaning, like humans they give birth to live babies.
Heterozygous mating produced Slitrk6+/+, Slitrk6+/−, and Slitrk6−/− pups at the expected Mendelian frequencies.
Pup vocalizations were recorded from 15 pups at p5, and from 14 pups at the subsequent ages p7, p9, p11 and p13.
These rates may still be underestimates because of the propensity of females to make brief visits to breeding areas other than their parturition site [2]; there were no data indicating that the females had pups at the harvest site.
Two days after H/I (P8) 2–5 mL of DAP or pNIT replication deficient retroviruses with 8 ug/mL polybrene were stereotactically injected at a rate of 0.2 µl/min targeting the lateral ventricles of anesthetized rat pups at the following coordinates: A 1.0 mm, L 1.2 mm, D 2.5 mm.
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No difference appeared in the mean birthweight and after the litter size was equilibrated to 11 12 pups at birth, the evolution of the mean litter weight was identical regardless the maternal diet until weaning (results not shown).
This, together with the fact that adult females spend 24% of their time ashore to nurse their pups at this time of the year [9], indicates offshore female mortalities were neither overt, nor overlooked.
Over all, the atmosphere around the rink is much different from nine years ago, when Messier first arrived from Edmonton, a mere pup at the age of 30.
Shorter treatment with nimesulide for 4 days increased only the time to delivery of the first pup at the 25-mg/kg dosage (P =.008).
Although John Dunning was only a pup at the time, there's a nice feeling of the here and now in the homage that he pays to the artistic ideals and technical craftsmanship of the fledgling industry in his bulky but hugely entertaining period mystery, TWO O'CLOCK, EASTERN WARTIME (Scribner, $26).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com