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Second, we conducted a classical conditioning paradigm in pups aged six to ten days.
For example, in very young pups, intake of milk diets is unaffected by MA, while pups aged 12 to 15 days demonstrate increases in intake.
In the first experiment, rat pups aged 18 days were injected with 0 or 68.4 mg/kg MA, then given 60-min tests while consuming either milk or chow diets that were novel, or to which they had previously been exposed.
As pups aged, the complexity of vocal bouts increased, with a greater tendency to switch between syllable types.
A common pattern was an initial increase in syllable duration between p5 and p7 or p9, followed by a decrease as pups aged further (Fig. 7A).
Male SD pups, aged 48 h±2 h, received STZ (Sigma, USA) injection intraperitoneally at a dose of 90 mg/kg body weight.
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As pups age, they produced a greater variety of syllables, with generally more complex features.
In vivo gene transfer was performed with Hp/− and control littermate Hp/+ pups, age 2 4 d.
Twenty-four neWeddelleddell seal pups (age 3-5 weeks, mean mass 75 ± 3 kg) were captured over three field seasons (October-December of 2002, 2005, and 2006) by using mild physical restraints or a purse string net near the pupping colonies in McMurdo Sound, Antarctica.
We injected naïve pups, age 6-8 withs, with cancer cell lines other than S180 initially to determine if resistance to these cell lines was simply a result of cross-vaccination from antigens shared with S180 or if it was a result of innate recognition of a common surface property shared between different cancer cell lines.
In 2002 we also collected 377 pups of the year (POY), out of which larger pups were aged using teeth, whereas seals shorter than 95 cm were regarded as pups of the year [17].
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