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vGRF was significantly higher during the traditional compared to modified PUP (p < 0.001), with a mean difference of 17%.
The final average KD pup weight at P20.5 was found to be significantly lower than that of the SD counterpart (p <0.0001), whereas the average adopted SD weight was similar in value to that of the average biological SD pup (p >0.05).
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As previously reported, ovaries from PND8 OE female pups contained 50% more primordial follicles than WT pups (P < 0.05).
Unlike WT pups, relative to vehicle controls, in utero exposure to VCH resulted in a reduction in primordial (25% of control), primary (38% of control), and secondary (33% of control) follicles in ovaries of OE pups (P < 0.05).
There was no difference in USV production between m−/p+ and m+/p+ pups (p = 0.82), or between m+/p− and m+/p+ pups (p = 0.3) recorded on clean bedding in cold temperature on day 10 and day 12.
Notably, Asic3+/− mice also spent less time than Asic3+/+ mice licking/grooming/sniffing the pups (p = 0.0003).
Expression was determined first in liver at the end of postnatal week 3. Significant changes in transcript levels were detected for Gnas (reduced in GLP pups; p = 0.036) and Grb10 (elevated in PLP pups; p = 0.025) (Fig. 1A).
The cumulative number of live pups born to BPA 25 ng/kg mice was significantly lower compared with controls starting at week 16 (mean ± SE, 49.74 ± 4.21 pups vs. 67.48 ± 4.38 pups; p = 0.037) and persisted until the end of the experiment (67.00 ± 5.70 pups vs. 81.90 ± 5.45 pups; p < 0.001).
HT reduced interleukin-1β (IL-1β) at all time points (p < .05); monocyte chemoattractant protein-1 (MCP-1) trended toward being decreased in HT pups (p = .09).09
Likewise, we observed a statistically significant difference between BPA 250 ng/kg and DES groups beginning at week 27 (86.11 ± 7.39 pups vs. 65.45 ± 6.62 pups; p = 0.037) and persisting until the end 88.222 ± 7.95 pups vs. 65.70 ± 6.66 pups; p < 0.004).
We observed a statistically significant difference between this group and controls beginning at week 16 (49.85 ± 3.69 pups vs. 67.48 ± 4.38 pups; p = 0.037) that persisted until the end of the experiment (58.25 ± 4.77 pups vs. 81.90 ± 5.45 pups; p < 0.001).
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