Moreover, for each value of PUP the cell response is analysed in terms of deformation as a function of time.
For each value of PUP about 40 60 HL60 cells are stretched to collect enough data for a statistical analysis.
Regardless of whether the mice were WT or hetero/homozygous mutant pups, we found cells that had incorporated BrdU in the hippocampal subventricular zone (hSVZ), fimbria and the DG (Figure 4A and B; [28]), suggesting that progenitors originating in the hSVZ divide while migrating towards the DG.
Highly enriched cultures of neocortical astrocytes were generated from P1 2 rat pup mixed glial cell cultures (Levison and McCarthy, 1991) and then maintained in a biochemically defined medium for 2 days to promote their differentiation.
However, the total thickness of the neocortex was significantly enlarged (17%; Figure 7G) when compared with control pups and the cells appeared to be more closely aggregated than in control.
Hilton et al. (2003) showed that females, rather than males, were more sensitive to kainic acid-induced hippocampal damage in rat pups, and female cells being significantly more sensitive to stressors such as ethanol- and camptothecin-induced cell death than male cells (Penaloza et al., 2009), documenting the sex-dimorphic cell response to death.
Briefly, cerebelli from 6- to 7-day-old pups were removed, cells were separated, and 2.0 × 10 cells/mL were seeded in poly-l-lysine (50 mg/L)–coated plates using basal medium Eagle (BME), supplemented with 10% fetal calf serum, 20 mM potassium chloride, and 1% penicillin-streptomycin and cultured at 37°C in 5% carbon dioxide.
For quantitative analysis of BrdU-immunoreactive cells, 2 pups from 4 litters were studied (8 pups each in C, LMH + T4, and LMH groups) and were counted in a total of 12 probes (600 μm wide; 1 2 probes per pup).
Cell surface Pup modification assay was performed in the same way as in (a).
Cell surface Pup modification assays were set up the same as in Fig. 6.
