On day 29, blood samples for bioassays were collected by cardiac puncture under diethyl ether anesthesia.
Blood samples of mice were collected by heart puncture under 20% urethane anesthesia on the day of sacrifice between 9 am to 11 am.
Blood sampling was performed by cardiac puncture under deep anesthesia at 30, 60 and 120 min after the mice were placed in clean cages.
The finite element program ABAQUS is used to conduct a parametric study on pipeline puncture under external interference loading by excavator teeth.
The animals were killed after 24 h by cardiac puncture under anesthesia with pentobarbitalnatrium.
The samples were collected by intracardiac puncture under general anesthesia (intraperitoneal injection of 250 mg/kg ketamine and 10 mg/kg xylazine).
The experimental results confirmed a high venous placement rate of 80% for puncture under the proposed conditions with the skin opened.
LUCAS II system (PhysioControl, Sweden) was used for chest compressions during ECLS insertion and cannulas were placed with percutaneous puncture under fluoroscopy or ultrasound control.
Samples were collected 6 h after surgery by intracardiac puncture under general anesthesia (intraperitoneal injection of 250 mg/kg ketamine and 10 mg/kg xylazine).
24 h after CCl4 injection, rats were sacrificed by heart puncture under deep ketamine/xylazine (2 1) anesthesia and liver tissue and blood samples were collected for further analysis.
They were sacrificed by cardiac puncture under ether anesthesia, and blood was withdrawn with a syringe and immediately placed in a pre-chilled EDTA-containing polypropylene vial on ice.
