There are a few punctual differences in sequences in the LTRs as compared to the pAP2-IRESeGFP 3' LTR.
Analysis of variants with substitutions or eliminations located in the 5′ or 3′ ends revealed that only in a few cases were sequence ends presenting punctual changes not detected directly by the LPS9.
Additional file 3 implements a most extensive detail of this pipeline and the corrections (punctual insertions/deletions) performed on each sequence.
Many copies even within the same family appear fragmented and divergent in nucleotide sequences due to several punctual mutations, rearrangements, and insertions or deletions (indels).
Punctual mutations were performed on the FAK coding sequence through directed mutagenesis (QuickchangeII XL site-directed mutagenesis, Agilent Technologies, Massy, France).
For a sequence of elements of and, denotes the punctual convergence, denotes the uniform convergence on compact sets, and denotes the uniform convergence, that is, the convergence in the generalized metric.
In addition, punctual glacier collapses would also have their imprint in the slackwater sequences with thicker, coarser and erosive sand deposits and the spring blocking by glaciers may have promoted floods inside the cave.
BR performed a systematic sequencing analysis of the GAN gene in patients and identified all punctual mutations.
Indeed, there is a nongradual process of molecular evolution at the origin of such composite genes, because both genetic fission and genetic fusion differ from punctual mutations, and may be responsible of larger, potentially more damaging changes in the sequences.
Tools have been specifically designed to distinguish between orthologous and paralogous in genome/proteome datasets [ 1] and expressed sequence tag datasets [ 2, 3], but they not always the most convenient for punctual analyses.
In order to understand the genetics underlying gene expression variation among A. thaliana accessions, we utilised the CATMA array technology [ 22], which has the advantage of not being sensitive to punctual polymorphisms (SNPs) between accessions' transcripts thanks to the length of the probes used (Gene-specific Sequence Tags: GSTs).
