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Using a leaf punch, leaf tissues from Agro-injected leaves were collected in 2-ml tubes and ground in liquid nitrogen.
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For lignin detection, leaf discs were punched from water- and thiamine-treated plant leaves, stained with phloroglucinol, and examined under a light microscope.
Because Nissan and GM have literally beaten Mitsubishi to the punch with their Leaf and Volt, Mitsubishi thought it a good idea to put as many press evaluation vehicles on the road as possible.
Approximately 0.5 cm (hole punch #4) of leaf tissue was put in a 55-mm plastic petri dish.
Punch the same leaf three times into the collection tube to ensure that enough tissue is obtained from each sample in the pool.
Here, the genomic DNA template for the direct PCR reaction was taken from a circular punch of dried leaf or fruit capsule material using a 0.35-mm Harris Uni-Core Micro-PuncHarrisrmo FisHarrisientific).
Leaf discs of 1-inch diameter were removed using a leaf punch about 15 cm from the tip of first and second fully expanded leaves, taking care to avoid the mid-vein.
After reaching a rosette size large enough to punch out several leaf discs of 1 cm diameter, they were brought to the experimental field site and exposed to natural, varying light conditions.
For verification of virus infection, each sample represented 3 inoculated leaves and 3 systemic leaves collected from each of the 3 repeated plants, with 3 leaf-discs (0.6 cm in diameter) punched from each leaf.
Ten days after inoculation, 8 mm leaf punches were randomly taken from eight N. benthamiana plants.
Two leaf punches were homogenized in 200 μL of each extraction buffer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com