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For whole brain homogenates frozen tissue was pulverized under liquid nitrogen.
Frozen tissue chunks were pulverized under liquid nitrogen, and homogenized in 10 vol of buffer (0.25 M sucrose, 1 mM EDTA [pH 7.4]).
Wound edge tissue [70] was pulverized under liquid nitrogen followed by extraction of protein in a buffer compatible with ELISA as described [77].
The frozen worms were pulverized under liquid N2 in a Spex 6750 Freezer Mill (Metuchen, NJ).
One ml of frozen cell aggregate was pulverized under liquid nitrogen.
Cryobanked specimens were pulverized under liquid nitrogen prior to nucleic acid extraction.
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The particle charging and conveying characteristics of pulverized coal under high-pressure dense-phase pneumatic conveying were investigated experimentally using CO2 and N2 as carrier gas, respectively.
Frozen tissue was pulverized into powder under liquid N2.
Tissue specimens were pulverized (Bio-Pulverizer™ BioSpec Products Inc., OK, USA) under liquid nitrogen and total RNA was isolated using Trizol reagent (Invitrogen Corporation, Carlsbad, CA, USA), according to the manufacturer's protocol.
Tumor specimens were pulverized (Bio-Pulverizer™ BioSpec Products Inc., Oklahoma, USA) under liquid nitrogen and total RNA was isolated using RNeasy kit (Qiagen, Valencia, CA, USA), according to the manufacturer's protocol.
The stability of pulverized coal flames under oxy-fuel combustion is one of the most critical issues in engineering practices.
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