Samples were sonicated for 30 minutes, with 30-second pulses, 1 minute resting, using the Bioruptor sonicator (Diagenode) to produce chromatin fragments of 0.5 kb on average.
However, they recommended slowing the shock wave firing rate from 120 pulses per minute to 60 pulses per minute to reduce the risk of renal injury and increase the degree of stone fragmentation.
This filter bank is able to detect tatum tempos in the range from 81 to 333 pulses per minute.
The range might need adjustments when very slow music is processed, that is, music with no tempo faster than 81 pulses per minute.
The air bubble was pulsated at a rate of 20 pulses per minute, between a defined minimum bubble radius of 0.4 mm and a maximum bubble radius of 0.55 mm (representing a 50% surface area change).
The peripheral pulse was strong and equal, with a rate of 140 pulses per minute (reference range 80 - 110).
We selected a rate of 60 pulses per minute, a baseline pressure of 80 mm Hg and a 'systolic' peak pressure of 110 mmHg.
In the latter two groups a vascular pump applied pulsatile pulmonary artery pressure to the motionless lungs at frequencies of 3 or 20 pulses per minute.
The mitochondrial Ca2 + retention capacity (CRC) was determined by measuring external Ca2 + following addition of 10 μM Ca2 + pulses each minute to medium containing 0.5 mg/ml of mitochondria.
The output of this circuitry was stored on data loggers (μlogger RVIP, Zeta-tec, England), one each for entry and exit, which counted the number of pulses per minute.
If the victim still has a pulse, continue rescue breathing (step 5), checking the pulse each minute (or 12 cycles).
