Sentence examples for pulse washed from inspiring English sources

Samples were collected during the pulse, washed three times with cold 5% trichloroacetic acid and the pellet is stored at -20°C.

Cells were then spun at 1500 r.p.m. for 10 min and the pellet was pulse washed with phosphate buffered saline (PBS, with calcium and magnesium) 5 to 10 times at 1500 r.p.m. for 2 s.

In this assay, cells were incubated with the compounds for 5 h (pulse), washed to remove unbound compound, and then incubated for an additional 67 h (chase) in fresh media.

They mostly materialize around stuff that he thinks might look cool or complement with a piece of music (as evinced by the pulsing Washed Out track).

Then, cells were incubated on ice with 0.5 mM Texas-red dextran (lysine fixable, Mr 3000; Molecular Probes) in the medium for 10 min (pulse) and washed 3 × with cold PBS.

After a 60 min pulse, cells were washed 3 times in PBS and then chased in DMEM containing excess cold methionine and cysteine (2 mM) for the time periods indicated.

At the indicated time post-pulse, cells were washed 1X in cold PBS and harvested in VSV G Lysis Buffer (50 mM Tris pH 8, 62.5 mM EDTA, 1% NP-40, 0.4% deoxycholate, 50 µM leupeptin, 200 µM PMSF, 1 µM pepstatin A).

After pulsing, cells were washed three times in PBS.

To produce LPS and TNF pulses, chambers were washed with media after incubation with ligand for the desired duration.

To visualize the specific endocytic uptake of GFP-GPI or TfR, cells were incubated with fluorescent conjugates of αGFP or Tf (along with fluid phase markers if needed) at room temperature for different pulse times and then washed in M1 and placed on ice.

Samples for analysis were taken at 8, 10, 12 and 16 h after the BrdUrd pulse, the cells were washed with PBS and fixed in 70% ice-cold ethanol.