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The most recent Clinical Practice Guidelines recommend measuring blood pulse to test for suspected AF, and performing ECG to confirm a diagnosis of AF [ 28].
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The SKA will time these pulses to test general relativity, Einstein's theory of gravity.
We have generated overlapping pulses to test how robust the proposed system is.
For measurements of ICa from ventricular myocytes, currents were evoked by 300 ms depolarizing pulses to test potentials between −40 and +60 mV (0.2 Hz).
In addition, the time pulse used to test the spatial selectivity to generate action potentials in simultaneous recordings of two interneurons was always at least the minimal time needed to elicit an action potential in the nearby non-photostimulated interneuron.
Current-voltage (I V) relationships were obtained by holding cells at a potential of − 98 mV (holding potential, HP) before applying 300-ms pulses to various test potentials.
Fluorescence voltage-relationships were derived by normalizing the ΔF/F value at the end of a four second test pulse to various voltages to the value of the highest voltage test-pulse.
The voltage-dependence of inactivation was assessed by applying a 50 ms test pulse to Vmax before and after holding cells for 5 s at various conditioning test potentials.
Steady-state fast inactivation was measured as the proportion of current remaining in a test pulse to 0 mV mV following 200 ms pulses to voltages between −130 mV and +10 mV.
From a holding potential of −90 mV, 100-ms prepulses were generated, ranging from −90 to 65 mV with 5-mV increments, immediately followed by a 100-ms test pulse to 0 mV.
Nav1.8 current was elicited by a 100-ms test pulse to 0 from a holding potential of −60 mV in a small-sized DRG neuron which exposed to the bath solution with 500 nmol/L TTX.
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