It can animate complex phenomena, like the formation of weather systems, the effect of global warming on wolverine habitats or the annual pulse of sea ice.
Cultures were centrifuged for 15 min in the dark at 4000 x g, resuspended with five mL of methanol containing 1%% butylated hydroxytoluene (BHT) and sonicated twice with 30s pulses on ice, at 50%% of output power using a Microson Ultrasonic Cell Disruptor XL2005 (Heat Systems, Farmingdale, New York) equipped with a tapered 3 mm microtip.
Mammalian cells were lysed in NP-40 Lysis buffers without EDTA, for bacterial cells; lysis in PBS occurred by sonication using 3 rounds of 5 × 1 seconds pulses on ice.
Immediately after the pulse, 1 ml of ice -cold 1M sorbitol was added and transferred into a tube containing 4 ml of YC-trp media for incubation at 30°C.
"We're at the pulse of the eternal ice".
Add three or four tbs. of iced water (no chunks of ice should be added) and pulse three to five times for 1 1/2 seconds per pulse.
Over the last two weeks, at a tenuous encampment at the very top of the world, a team of researchers, coping with frigid winds and solar storms that cut radio links with the outside world, went into high gear on an ambitious five-year effort to take the pulse of the polar air, ice and ocean depths.
In January , 1930 Gernsback published "The Corpse That Lived": a man who dies in a plane crash in the year 2025 is immersed in a bathtub of ice cubes and brought back to life by an electric pulse.
The altimeter system, measuring the time it takes light pulses to travel to a reflecting object and back to the satellite, will calculate the precise elevations and positions of ice sheets, icebergs, clouds and land formations.
Greenland ice cores record a pulse of starkly reduced levels of organic carbon sequestration.
