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Non-embryogenic callus developed from the skin and pulp sections of the explants (berry slices) within three to six weeks of culturing.
Specificity studies of the Nav1.8 antibody (K107), showing positive staining in human DRG neurons, and no staining in pre-absorption experiments using Nav1.8 peptide in tooth pulp sections, were performed as previously described [ 9].
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The aim of this study is to select the most appropriate technologies in the pulp section, due to high impact of pulp quality on the quality of final paper.
For assessment of lesion depth, the subsequent examiner marked the base of the lesion and the corresponding pulp section using the brush tool at a fine setting.
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The use of engineered CBMs in future studies will further expand the potential of these types of synthetic probes for mapping of pulp fibre and wood sections.
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