Sentence examples for pulldown in from inspiring English sources

This negative control was done in parallel with the pulldown in Figure 6C.

We next investigated whether our peptidic probe could achieve selective affinity pulldown in a more complex system.

We have performed the RBD RAB-3 pulldown in snt-1 rbg1 snt-1 rbg1ants andoublecorresponding data has been included in Figure 4C.

Second, ATF7IP is implicated to interact with CDYL, a chromodomain-containing transcriptional co-repressor that is recruited to the Xi by H3K9me2, as ATF7IP was recently identified by mass spectrometry in a CDYL pulldown in undifferentiated and differentiating mouse ESCs [ 27].

Protein interactions were tested in two-hybrid, GST pulldown, semi-in vivo pulldown and Tandem Affinity Purification assays.

The low abundance of these co-precipitating bands compared to that seen in immunoprecipitations of the RON complex in AMA1 pulldowns in Toxoplasma likely reflects the more stringent RIPA conditions used here compared to the lower salt and detergents used to isolate the moving junction complex in previous studies in Toxoplasma [32], [58], [59].

One approach would be to examine RBD RAB-3 pulldowns in snt-1; rbg-1 double mutants.

Consistent with this observation, Golgi SNARE complex pre-bound to Sec17 and then washed retained less Sec17, and commensurately less Sly1, versus pulldowns in which Sec17 was in excess.

Three of these 16 were annotated as "putative proteins" in SGD, whereas the other 13 genes showed evidence for the existence of a protein product using methods such as electrophoresis/chromatography separation followed by mass spectrometry, detection of tagged pulldowns in yeast expression systems, and/or documented catalytic activity of the purified form.

Fifty-seven percent of the interactions revealed by our YTH analysis was confirmed in co-IP experiments, consistent with previous observations that about 50 60% of the YTH interactions are positive in immunoaffinity pulldown experiments in the studies of other viruses [21], [22], [23], [24], [31].

Wild type and mutant FLAG-tagged ADNP proteins were expressed in TNT reticulocyte extract and used in H3K9me3 peptide pulldown experiments in conjunction with recombinant HP1β.