Exact(6)
Con (control pulldown from HEK mitochondria).
(C) RNASET2 was purified from RNASET2-overexpressing mitochondria under denaturing condition and checked for ribonuclease activity using RNA purified from isolated mitochondria as substrates; C (control pulldown from HEK mitochondria) and T2 (RNASET2).
To ensure the specificity of ChIP using the anti-CaRF antibody, we sequenced ChIP libraries made from both Carf WT and KO neurons then developed a statistical method to identify only those genomic regions significantly enriched in the pulldown from the WT cells.
(E ) Immunoblots of GST-TBK1 ULD pulldown from 3T3-L1 adipocytes.
(B ) Immunoblots of GST-14-3-3 GST-14-3-3 GST-14-3-3 GST-14-3-3xpulldown HA-PDE3B or HA-PDE3B S318A with from-TBK1.
(E ) Immunoblots of GST-14-3-3 GST-14-3-3 GST-14-3-3 GST-14-3-3xpulldown HA-PDE3B with from-TBK1 or Flag-TBK1 K38A.
Similar(54)
To independently confirm the results of the MS analysis, we repeated the H3 peptide pulldowns from HeLa S3 nuclear extracts.
FLAG-pulldowns from untreated cells or from each timepoint after UV-irradiation, were probed with antibodies specific for either FLAG, endogenous DDB1, endogenous emerin or endogenous BAF (Figure 4B).
For pulldowns from extracts, nuclear-enriched fractions were isolated using an established method [ 38].
TED proteins for cell binding assays and pulldowns from plasma were expressed from pDEST-iPT.
The L antisense RNA region is enriched in LGP2 pulldowns from EMCV-infected cells.
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