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However, Noc treatment may also increase the depolymerization rate of microtubules in contact with the cortex and hence increase forces that may pull the spindle apically.
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This occurs through the concerted effort of dynein dynactin directed microtubule transport with the help of dynein-associated proteins such as CLASP1, Lis1, and Nde1/Ndel1, thereby pulling the spindle into position [ 14 17].
We tentatively speculate that CBX1 is required for some aspect of kinetochore or chromokinesin function that normally helps pull the two half-spindles together.
The CENP-ACnp1 negative centromere was not pulled to the spindle pole in anaphase, which is consistent with centromere inactivation.
Sister chromatids are then pulled towards the spindle poles, followed by cleavage furrow formation and ingression, culminating in the physical separation of cells during cytokinesis.
DOI: http://dx.doi.org/10.7554/eLife.01861.002 Centromeres are the genetic loci that organize the proteinaceous kinetochore, which attaches to spindle microtubules to pull the chromosomes to the poles in both mitosis and meiosis.
The ParABS system has been proposed to be analogous to the eukaryotic spindle-based mechanisms using a filament of ParA to pull the ParB/ parS complex along.
In the cells of plants, animals, and fungi, a structure known as the spindle pulls the two copies of the chromosomes apart.
Once the chromosomes are in place, the checkpoint is turned off and the spindle pulls the chromatids apart so that each daughter cell receives a complete set of chromosomes.
At a concert Monday at the Bell Centre in Montreal, the 31-year-old's hair was pulled into the spindles of the rogue stage fan during her performance of "Halo".
To achieve this, protein complexes called kinetochores connect the chromosomes to a structure called the spindle, which pulls the chromosomes to opposite ends of the cell.
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