Exact(21)
In the absence of plasminogen, the bead pull down of cell wall protein extracts failed to yield detectable protein.
Pull-down with protein-A Sepharose lacking anti-OVA IgG led to limited (a-specific) pull down of ApoB-48.
However, hDAT N64 pull down of STX1ΔTM S14A was dramatically blunted.
(C ) Pull down of importin α from stage 3 or stage 8 embryo extracts by recombinant kif2a.
In Figure 2E, pull down of RanGTP by GST-Slx9 or GST-Slx9-1 ishownwn, and Slx9-1 has a modestly reduced interaction compared to Slx9.
Thus, our results exhibit a stark dissociation between inter-CaV2.2 scaffolding, as indicated by C terminal pull down of channel, and RIM1/2 capture.
Similar(39)
We further confirmed a physical association between Prdx1 and PTEN by using GST-pull down of GST Prdx1 and His PTEN.
Indeed, pull-down of HDL nanodiscs from human serum allows for the recovery of HDL-associated LAM.
RhoA activities were determined by pull-down of GTP-RhoA.
Blotting with an HA-antibody confirmed specific pull-down of Grb2 by Dvl2 (Fig. 1B).
Kd determinations by Tpm1-Gel 10 pull-down of purified Cof1 were performed as described [25].
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