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The protein was pull down by GST tag, then GST was cleaved off and the protein was further purified with gel filtration chromatography.
I will pull down by baseball hat even lower and tell my girls my allergies are acting up again.
Its pull down by specific antibodies can therefore improve the sensitivity of the enrichment process.
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Corn Is Pulled Down by Wheat.
Protein-antibody complexes were pulled down by adding beads to the solution for 2 hr.
The protein antibody complexes were pulled down by adding protein A/G-Sepharose beads.
For immunoprecipitation, the HSF1 containing protein complexes were pull-down by anti-HSF1 antibody, washed and eluted from beads via boiling and subjected to immunoblot using anti-ubiquitin antibody (Enzo life sciences).
Proteasomes affinity purified from rpn13Δ cells were successfully pulled-down by all three baits.
After incubation, the protein complex was pulled-down by Ni-TED silica beads.
Moreover, BT was functionally toxic and successfully pulled down by NeutrAvidin in vitro and in vivo.
25 years ago the Berlin Wall was pulled down by jubilant East Germans.
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