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Numbers in parentheses are NCBI accession numbers of published sequences.
Sequences were compared with published sequences from the public sequence databases.
For the methylation analysis, the primers for the three Igf2/H19 DMRs were designed and based upon previously published sequences.
We show why and under which circumstances a literal adoption of phases from published sequences can lead to incorrect results.
This species was also clearly distinguishable from all published sequences of Gracilariaceae in the COI Neighbor-Joining analyses (Figure 2).
Bands G and K were closely related to published sequences classified in phylum Firmicutes, family Peptococcaceae, and genus Desulfosporosinus.
Where possible, we integrated published sequences of numerous atyid species into our datasets (Table 2).
Real-time PCR primers were either based on published sequences or designed using VectorNTI (Invitrogen).
Those products were sequenced and compared to published sequences in GenBank via BlastSearch [27].
The published sequences of Remigio and Hebert [12] for single specimens of L. h.
Using this protocol, 158 previously published sequences were added to the alignment (File S1).
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