Here, we report an improved method yielding highly pure, copper-free methanobactin extending the published one-step resin extraction method with a subsequent purification step with HPLC using a reversed-phase C18 column.
The CoV screening was performed by amplifying a 179-nt stretch of the CoV RdRp gene using our previously published one-step pan-CoV RT-PCR method [24].
To demonstrate the superior sensitivity of the two-step approach, the lyssavirus qRT-PCR was compared with a previously published one-step lyssavirus qRT-PCR [ 33].
Equations were rederived step by step, and for some of them we obtained results different from the published ones.
After publishing one leaflet on safe sex the Catholic Herald demanded he be banned from stepping foot in Ireland.
We need to start thinking around these issues today because - thanks to AOL - the mass amateurisation of publishing is one step nearer.
Reedsy has been unbundling traditional publishing houses one step at a time.
The photos on its Flickr feed are now licensed CC-BY, the least restrictive license Creative Commons publishes, and just one step away from being fully public domain.
Genome equivalents in serum and homogenized tissues were determined using a previously published TaqMan one-step RT-qPCR assay targeting the genomic fragment encoding the nonstructural protein 551.
