The choice to use an acidic pH was based on the published conditions reported [16], [17] for both of the experimentally determined structures of the full length peptide (PDB entries 2DX2 and 1CB3).
Previously published condition-specific systematic reviews and meta-analyses [ 39, 42, 45] were used to guide the development of the search strategy and identify pertinent publications.
Samples were amplified and genotyped using published conditions [ 105].
Nocturnal light intensities mimicking "full moon" were between 1-10lux according to previously published conditions (Hauenschild, 1960 ).
The mtDNA control region was PCR amplified using primers L15996 and H408 [ 16] following previously published conditions [ 17].
Stem-loop RT PCR (TaqMan MicroRNA Assays; Applied Biosystems) was used to quantitate microRNAs according to the earlier published conditions (Ichimi et al, 2009).
We extracted genomic DNA using a 5% Chelex 100 solution (Bio-Rad Laboratories, HerCAles, CA, USA) for use as templates in PCRs using previously published conditions (Savage and Jaeger 2009).
Stem-loop RT PCR (TaqMan MicroRNA Assays; P/N: PM10617 for miR-1, and PM10413 for miR-133a; Applied Biosystems) was used to quantitate miRNAs according to the earlier published conditions (Ichimi et al, 2009).
DNA from individual samples was prepared as described previously [ 49] and analyzed for their genotype across the GPC-B1 interval using markers Xuhw89 (distal), Xucw71 (proximal) and Xucw101 (causal SNP at GPC-B1) using published conditions [ 11, 14].
Ready-to-inject, >99% radiochemically pure L-[C]methionine ([C]MET, (S -2-amino-4- [C]methylsulfanyl -butanoic acid) waS -2-amino-4- [C]methylsulfanyl -butanoicbon dioxide (Cyclone 18/9 cyclotron, IBA, Louvain-la-Neuve, Belgium) on the baS -2-amino-4- [C]methylsulfanyl -butanoic a commercially available TRACERLab™ FX-C S -2-amino-4- [C]methylsulfanyl -butanoic
