These structures have all been published by others using the two assays shown here, but there is no real incorporation of the MP cells into nephron-like structures.
The data published by Henderson and Johnson using a DAF-16::GFP fusion transgene suggest that in adults under standard laboratory conditions without starvation, the nuclear localisation of DAF-16 is not sufficient to discern nuclei.
This is tree in good agreement with the phylogeny published by Gao and Gupta using 35 conserved genes from 98 actinobacterial genomes [ 26], although there are a couple of notable differences.
Terminal velocity analysis was found to have good agreement with previously published data by using its (terminal velocity) true value.
The effectiveness of the bi-population strategy and local search procedure is shown by numerical comparisons, and the comparisons with the recently published algorithms by using the benchmarking instances also demonstrate the effectiveness of the proposed BEDA.
However, we believe that we have captured the peer reviewed published literature by using both PubMed and IndMED.
Additionally, ORGENOTYPE was meta-analyzed with published studies by using fixed effects analysis given the lack of heterogeneity between studies.
The design of siRNA was performed according to the previously published guidelines by using the Ambion siRNA-finding software [ 14].
The S gene nucleotide sequences and deduced amino acid sequences of the 3 new isolates were aligned with the sequences of published isolates by using MEGA 4.0 (www.megasoftware.net).net
After purification these PCR products were sequenced with an ABI 377 automated sequencer, and the sequences were compared with previously published sequences by using the BLAST program from the National Center for Biotechnology Information Web site (available from: http:/ www.ncbi.nlm.nih.gov/BLAST/).nih.gov/BLAST/
