Exact(3)
Genotypes used in this study included 38 individuals from a Chardonnay × Bianca pseudo test cross [ 20] and a set of diverse grapevine genotypes.
The genetic maps of the four parents of the two populations were constructed using double pseudo test cross methodology [ 55] and JoinMap v3.0 software [ 56] based on the SNP data for the individuals in each population.
The heterozygous nature of grape cultivars, makes linkage mapping to be performed in F1 populations and maps are constructed for each of the parental genotypes using a pseudo test cross strategy [ 54- 59].
Similar(57)
In studies where subjects could select either such pseudo-tests or genuinely diagnostic ones, they favored the genuinely diagnostic.
For genetic map construction, we followed the two-way pseudo-test cross approach [ 38].
For linkage analysis, the double pseudo-test cross strategy was applied.
In constructing the map, the double pseudo-test cross strategy of Grattapaglia and Sederoff [ 63] was applied, using JoinMap4.0 (Kyazma).
In addition, in a "two-way-pseudo-test-cross" mapping strategy, intercross markers i.e. markers with the same heterozygous allelic configuration in both progenitors, are less informative.
Until recently, mapping procedures in autopolyploids plants were based on SDRF (single-dose restriction fragments) segregation in mapping populations analysed as pseudo-test crosses [ 10, 11].
We constructed 10 parental genetic linkage maps (7 for Q. robur and 3 for Q. petraea) by the two-way pseudo-test cross mapping strategy [ 30].
Grattapaglia and Sederoff [ 7] proposed a so-called pseudo-test backcross strategy for linkage mapping in a controlled cross between two outbred parents.
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