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The procedure of linear discriminant analysis is to find a linear combination (the linear discriminant function, LDF) of these characteristics, that provides maximum discrimination between real and pseudo sites.
Thus, these sites were classified as pseudo sites (as Pseudo. in Figure 1B).
phiC31 integrase has also been used for integration into endogenous "pseudo sites" in certain cellular environments, however it does not appear to be universally capable of this reaction (Chalberg et al., 2006; Groth et al., 2004; Ma et al., 2006; Ni et al., 2012; Olivares et al., 2002).
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The third site, designated as pseudo site 3, constitutes a large interface between Wnt3a and a second CRD.
Janda et al. have in addition reported a third contact site in the crystal structure designated as pseudo site 3, which was speculated to mediate asymmetric Wnt/Fz dimerization and thus self-assembly to polymers.
We also show that BC hybrids can function efficiently in HeLa cells, in both extra-chromosomal and pseudo site assays.
Mutations in pseudo site 3 either abolished or enhanced Wnt3a signaling, suggesting a dual role in signal modulation.
In line with its parental enzymes, we found that BC{−1} integrase was able to carry out pseudo site integration in HeLa (Fig. 6D).
The model had class terms for treatment (five levels), site (sites with less than ten patients were combined into a pseudo site), and a covariate term for baseline ADHD-RS-IV total score.
Recognition of both pseudo site types may preclude usage of BC{−1} for genomic engineering due to safety concerns, as recombination between said sites could lead to unwanted inversions, deletions and/or translocations.
To detect pseudo site integration, we co-transfected different combinations of G418-resistance donor plasmids, which carry an att-site (Fig. 6A C), with integrase expression vectors, and then counted the resistant colonies present after two weeks of G418 selection.
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