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32P-labeled DNA probes used to detect each specific mRNA were designed to only span the non conserved region of each pseudo response regulator gene.
Two of them, PtRR27 and PtRR29, have an atypical conserved motif (HD-D-K and DD-E-K, respectively), and may represent pseudo response regulators.
In the morning loop, the two MYB-type transcription factors CCA1 (CIRCADIAN CLOCK ASSOCIATED 1) and LHY (LATE ELONGATED HYPOCOTYL) activate two pseudo response regulators, PRR7 and PRR9 that feed back to repress CCA1 and LHY [ 10- 12].
Two members of the pseudo response regulator gene family, PRR7 and PRR9, repress CCA1 and LHY during the day, and this establishes the morning loop (Locke et al., 2006; Zeilinger et al., 2006).
Differential expression of genes associated with cytokinin signaling included regulation of cytokinin histidine kinase receptors, two phosphotransfer proteins, and A-, B-type, and pseudo response regulators.
In this study we also characterized two late auxin-responsive genes regulated independently of CRL1: OsMADS (MADS- BOX INTERACTING PROTEIN1) and OsPRR95 (PSEUDO RESPONSE REGULATOR95).
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In the morning loop, CCA1 and LHY promote the transcription of PSEUDO- RESPONSE REGULATOR 9 (PRR9) and PRR7 genes [ 8, 9].
Salomé, P. & McClung, C. R. PSEUDO-RESPONSE REGULATOR 7 and 9 are partially redundant genes essential for the temperature responsiveness of the Arabidopsis circadian clock.
Nakamichi, N. et al. PSEUDO-RESPONSE REGULATORS 9, 7 and 5 are transcriptional repressors in the Arabidopsis circadian clock.
In particular, early detection of treatment response by standard anatomical imaging is confounded by pseudo-response or pseudo-progression.
Turner, A., Beales, J., Faure, S., Dunford, R. P. & Laurie, D. A. The pseudo-response regulator Ppd-H1 provides adaptation to photoperiod in barley.
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