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These blotches contain asexual (pycnidia) and sexual (pseudothecia) fructifications.
It was obtained as a single-ascospore isolate ejected from a pseudothecia formed on Brassica juncea cv.
Inocula are asexual spores (pycnidiospores) released from pycnidia found on leaves and sexual spores (ascospores) released from asci contained in fruiting bodies (pseudothecia) found on infected leaves and plant debris (Supplementary Fig. 2G).
Pseudothecia were harvested 20 to 25 days after crossing, and single ascospores were isolated from the asci and allowed to germinate on PDA.
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