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In order to obtain the accuracy and precision of the ILS method, several synthetic mixtures with different concentration ratios of PA, MP, PP, CH and PS were analyzed using the proposed method.
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Total dissolved N and P were analyzed using persulfate digestion techniques from Valderrama [ 36].
The P NMRexperimental data versus pH were analyzed using the HypNMR 2006 software.
All identified putative signal peptides (SPs) were analyzed using the Signal P 3.0 server [ 68].
Concentrations of K were analyzed using a flame photometer and P was analyzed using the colorimetric method with molybdenum blue and ascorbic acid as the reducing agents.
In order to confirm that electrochemical signals were due to nucleic acid hybridization and conformational changes rather than other unattributed effects, the behavior of Probe P was analyzed using native polyacrylamide gel electrophoresis (PAGE).
Two hydroxycinnamic acids, ferulic acid and p-coumaric acid, and their respective benzaldehydes, vanillin and p-hydroxybenzaldehyde, were analyzed using reverse-phase HPLC.
P-values were analyzed using Mann Whitney U-texceptexcept for χ 2 tests.
The mean, s.d., s.e.m. and P-values were analyzed using the Student's t-test.
C p values were analyzed using the Light Cycler 480 application and fold changes calculated in Microsoft Excel.
Variables that showed an association at a significance level of P <.1 were analyzed using multivariate logistic regression analysis; differences were considered significant at P <.05.
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