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Polystyrene (PS) buffer layers were deposited by drop-cast method onto the substrates surface to ensure the adherence of the pure TiO2/PS and TiO2-GO/PS composite layer.
Random groups of 5 or more mice were treated iv with 5 µg/mouse of ricin in PS buffer.
In the oral model, 10 mice were treated with 1 mg ricin in 200 µl PS buffer via gavage using round-ended Popper needles.
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NadA, LOX-1 and the complex mixed in a 1 1 molar ratio were diluted in HBS-P buffer in a final concentration of 5 μM.
For kinetics analysis of the interaction between CRT (>90% pure) and immobilized ITGA4 peptide, 40 μL of various concentrations of CRT in HBS-P buffer were injected at a flow rate of 20 μL/min.
The isolated RCs were slurry suspended in a 0.01-M Na-P buffer, pH 7.2, with 0.05% LDAO [8].
Separated RCs were suspended in a 0.01-M Na-P buffer with a pH of 7.2, which contained 0.05 % LDAO [18].
Cells were washed once with 10.3% sucrose and resupended in P buffer containing 2 mg/ml lysozyme [34].
The running buffer was HSB-P buffer (10 mM HEPES [pH 7.4], 150 mM NaCl, 0.005% polysorbate 20 adjusted to 1 mM MgCl2) (BIAcore).
Injections on the anti-Fc coated CM5 chip were done at a flowrate of 5 µl/min and the different proteins were diluted in HBS-P buffer.
During Biacore studies, the chips were submitted to continuous flow of HBS-P buffer (10 mM Hepes, 0.15 M NaCl, and 0.05% P20 detergent, pH 7.4), and all injected material were diluted in the same buffer.
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