Caecal and distal pools of propionate were higher in HAMS than LAMST or LAMS groups (P < 0.05 0.001), and proximal pools of propionate were higher in HAMS than LAMST (P < 0.001).
During UUO, changes in both the frequency and amplitude of pyeloureteral contractions, in conjunction with sustained urinary pooling proximal to the site of obstruction, contribute to mechanical stretch injury of the tubular epithelium.
23 The two consequences are increase in archive size and availability of telomere-proximal VSG, possibly increasing the pool of telomere-proximal VSG that can interact readily with the expression site (switching) or with each other (recombination-mediated diversification).
The increased success was not a result of a steady performance increase over the course of the ten sessions of the two experiments (proximal and distal cues pooled: Pearson correlation: N = 10, r = 0.551, P = 0.215).
Under these conditions, Cts were observed to range from 18.2 to 38.5, and based on the results, primer pairs with proximal Ct values were pooled together, taking care to ensure that primers for overlapping regions were separated.
Based on these results, primer pairs with proximal Ct values were pooled together, taking care to ensure that primers for overlapping regions were separated, which resulted in 59 amplicons spread across 8 pools (five pools of 8, two pools of 6, and one pool of 5).
The pro- and mesothoracic legs of approximately 20 pupae were bisected at the tibia−tarsus joint to generate separate pools of proximal and distal tissue for RNA extraction using Trizol (Invitrogen/Life Technologies) followed by DNase I treatment to remove genomic DNA.
These data are consistent with the ascorbate pool within the proximal airway being largely determined by its uptake into goblet cells.
Per time point, equal amounts of total RNA of six mice per diet group were pooled for the proximal, middle and distal part of the small intestine.
