The heterodimerization of LIFR and gp130 triggers the membrane-proximal cytosolic docking and activation of the associated Janus kinases (JAKs) (Lütticken et al., 1994; Stahl et al., 1994).
Do proximal and distally docked SVs represent functionally distinct populations of SVs? Do they mediate different forms of neurotransmitter release or different forms of synaptic plasticity?
We found that unc-13 e1091) unc-13 e1091nc-10 RIM mutations made evoked release slower and more loosely coupled to calcium entry, both indicating that SVs docked proximal to the dense projection are required for fast release.
Analogous to the canonical pY-SH2 domain binding, the STAT5 pY likely docks proximal to the conserved R618 (βB strand), making H-bonding/electrostatic interactions with nearby polar residues, K600 (αA), T628 (βC), and S622 (βB and βC) Figure 2A.
By contrast, docking of proximal SVs was significantly reduced in unc-13 e1091) unc-13 e1091ts (<100 nm from dense projections) and in unc-10 RIM mutants (<50 nm from dense projections), whereas docking of distal SVs was unaffected in both mutants (Weimer et al., 2006; Hammarlund et al., 2007; Gracheva et al., 2008).
The types of transport through healthy tissue were descending (proximal distal) in two cases (femurs), ascending in four cases (one femur, three tibias), double transport in two femurs (with mid-diaphyseal contact of transported bone ends at the docking site from proximal and distal metaphyseal osteotomies), and in one tibia (twin transport from a double proximal osteotomy).
A new crystal structure of a kinesin-14 point mutant [ 1] now visualizes for the first time the docking of the proximal part of the kinesin-14 carboxyl terminus to a site on the main part of the kinesin head, showing that the docking and undocking of a carboxy-terminal peptide is a general feature of the mechanism of force generation in both plus-end-directed and minus-end-directed kinesins.
In addition, membrane-proximal tyrosine residues act as docking sites for molecules involved in the activation of extracellular signal-related kinase (ERK).
For example, in myoglobin (Mb), a transient docking site on the proximal side of the heme is readily populated by CO but not at all by NO.
These observations immediately suggest that a proximal, membrane-bound proteinase could easily dock to this complex.
The later is an essential component of the TCR-proximal signalling cascade, serving as the docking site for Vav1.
