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A proximal cluster (P) is located around map coordinates 110 and is contained within fragments that activate dpp along the entire A/P boundary.
An 800 bp fragment containing both the activating region (A), and the proximal cluster of dTCF sites (P) [(BS3.1, AP, ) [19]; 109.5–110.3] activates transcription along the A/P boundary but does not exhibit ventral repression (Fig. 1F).
As described earlier, the two dTCF sites in the Proximal Cluster of the APRD fragment are not sufficient to cause measurable repression when the distal complex is absent nor are TCF sites required for activation since fragments with all TCF sites mutated still drive expression (not shown).
This proximal cluster comprises an unusual [4Fe 3S] structure stabilized by six conserved cysteine residue ligands [ 21, 22].
By contrast, standard O2-sensitive [NiFe]-hydrogenases have a [4Fe 4S] cubane as a proximal cluster and rapidly form an inactive Ni-A 'unready' state upon O2 attack.
Such a conformational change would not be unusual or unreasonable to predict, and this could push His closer to the proximal cluster, or indeed pull it further away.
Similar(35)
The locus is organized into two distinct clusters, a constant (C -proximal C -proximaltaining IGLV3 family members, and a clusterl containingntaIGLV3 IGLV8 and IGLV5 family members [ 56].
We used the fact that the average width (four times the standard deviation) in perpendicular direction to the septum of the forespore- and mother cell-proximal cluster from dual-foci was 57 nm and 52 nm, respectively.
This is because the frequency with which genes in internal (>50 kb from telomeres) starvation-induced clusters are acetylated in hda1Δ compared to wild type cells is similar to telomere-proximal clusters of genes (57% of genes in internal clusters vs. 59% in telomere-proximal clusters).
Functional study using both cultured mammalian cells and chick neural tubes suggested that multiple CK1/GRK2 phosphorylation sites regulate mSmo activity in a dose-dependent manner, with the two membrane-proximal clusters (S0 and S1) playing a major role.
Ligands of the proximal [4Fe4S] cluster are in yellow, of the medial cluster in orange, and of the distal cluster in red.
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