Selective motor control, alignment and muscle strength were assessed in provoked functional activities against gravity e.g. in different positions on a Bobath ball, repeated sit-to-stand and squatting.
Analysis of genome-wide miRNA microarray demonstrate that a single miRNA can regulate hundreds of genes, but the regulative extent on most individual genes is surprisingly mild so that it is difficult to understand how a miRNA provokes detectable functional changes with such mild regulation.
With its multi-target characteristics, miR-181b, miR-34a and miR-20b provoked detectable functional changes by co-regulating functionally-related gene groups or several genes in the same signaling pathway, and thus mild regulation from individual miRNA targeting genes could have contributed to an additive effect.
Our data suggests that miR-181b, miR-34a and miR-20b co-regulate a group of functionally related genes to provoke detectable functional changes under certain condition.
Co-regulation of a group of functionally related genes to provoke detectable functional changes is a common mode of miRNA-mediated gene regulation.
Taken together, miR-181b, miR-34a and miR-20b may have provoked detectable functional changes by co-regulating functionlly related gene groups or several genes in the same signaling pathway.
Anderson et al. (2012) recently identified how crowded stimuli provoked increased functional MRI activation from areas V1 V4, also suggesting that crowding may be a multistage process.
Taken together, these results indicate that there is a switch in Wnt ligand expression pattern in breast cancer cells, and that this may provoke a functional switch in Wnt signaling from non-canonical to canonical pathways.
Most seemed to result in a small shortening of the N or C terminal part of the proteins by only a few aa which may presumably not provoke major functional modifications (Table S6), for example in the case of Solyc01g058160, Solyc01g095620 or Solyc09g082630 genes in L5 (Table 2).
